Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus.
نویسندگان
چکیده
The nor-1 gene is involved in aflatoxin biosynthesis in Aspergillus parasiticus and was predicted to encode a norsolorinic acid ketoreductase. Recombinant Nor-1 expressed in Escherichia coli converted the 1' keto group of norsolorinic acid to the 1' hydroxyl group of averantin in crude E. coli cell extracts in the presence of NADPH. The results confirm that Nor-1 functions as a ketoreductase in vitro.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 65 12 شماره
صفحات -
تاریخ انتشار 1999